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                人鐵蛋白(FE)酶聯免疫吸附檢測試劑盒
                ELK1214
                規格: 價格:
                48 T ¥1680.00
                96 T ¥2400.00

                Overview 文獻

                Product name: Human FE(Ferritin) ELISA Kit
                Reactivity: Human
                Alternative Names: Ferritin
                Assay Type: Sandwich
                Sensitivity: 0.061 ng/mL
                Standard: 10 ng/mL
                Range: 0.16-10 ng/mL
                Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
                Assay Length: 3.5h
                Research Area: Metabolic pathway;Tumor immunity;Hematology;
                Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin(FE). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ferritin(FE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin(FE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin(FE) in the samples is then determined by comparing the OD of the samples to the standard curve.

                標準曲線

                Concentration (ng/mL) OD Corrected OD
                10.00 1.998 1.909
                5.00 1.639 1.550
                2.50 1.093 1.004
                1.25 0.855 0.766
                0.63 0.479 0.390
                0.32 0.356 0.267
                0.16 0.173 0.084
                0.00 0.089 0.000

                精密度

                Intra-assay Precision (Precision within an assay):CV%<8%

                Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

                Inter-assay Precision (Precision between assays):CV%<10%

                Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

                回收率

                Matrices listed below were spiked with certain level of recombinant FE and the recovery rates were calculated by comparing the measured value to the expected amount of FE in samples.
                Matrix Recovery range Average
                serum(n=5) 87-99% 93%
                EDTA plasma(n=5) 82-98% 90%
                Heparin plasma(n=5) 90-107% 98%

                線性

                The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
                Matrix 1:2 1:4 1:8 1:16
                serum(n=5) 95-106% 85-101% 87-102% 89-95%
                EDTA plasma(n=5) 88-97% 86-92% 91-99% 79-97%
                Heparin plasma(n=5) 85-104% 79-92% 83-95% 86-97%
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