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                人鐵調素(Hepc)酶聯免疫吸附檢測試劑盒
                ELK8188
                規格: 價格:
                48 T ¥2660.00
                96 T ¥3800.00

                Overview

                Product name: Human Hepc(Hepcidin) ELISA Kit
                Reactivity: Human
                Alternative Names: HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
                Assay Type: Sandwich
                Sensitivity: 0.61 ng/mL
                Standard: 100 ng/mL
                Range: 1.57-100 ng/mL
                Sample Type: serum, plasma and other biological fluids
                Assay Length: 3.5h
                Research Area: Metabolic pathway;Endocrinology;Hematology;Genetic science;
                Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin(Hepc). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepcidin(Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepcidin(Hepc), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepcidin(Hepc) in the samples is then determined by comparing the OD of the samples to the standard curve.

                標準曲線

                Concentration (ng/mL) OD Corrected OD
                100.00 2.172 2.099
                50.00 1.700 1.627
                25.00 1.136 1.063
                12.50 0.875 0.802
                6.25 0.548 0.475
                3.13 0.354 0.281
                1.57 0.196 0.123
                0.00 0.073 0.000

                精密度

                Intra-assay Precision (Precision within an assay):CV%<8%

                Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

                Inter-assay Precision (Precision between assays):CV%<10%

                Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

                回收率

                Matrices listed below were spiked with certain level of recombinant Hepc and the recovery rates were calculated by comparing the measured value to the expected amount of Hepc in samples.
                Matrix Recovery range Average
                serum(n=5) 87-95% 91%
                EDTA plasma(n=5) 78-93% 85%
                Heparin plasma(n=5) 87-99% 93%

                線性

                The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepc and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
                Matrix 1:2 1:4 1:8 1:16
                serum(n=5) 83-95% 88-102% 85-97% 80-92%
                EDTA plasma(n=5) 89-102% 92-101% 88-102% 87-102%
                Heparin plasma(n=5) 85-94% 87-101% 89-99% 85-95%
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